Michael Hooker Microscopy Facility (MHMF.ORG)

Hardware Resources

• FLUORESCENCE MICROSCOPES (Wide field)
  • Inverted Leica fluorescence microscope with digital B/W and color cameras and DIC
  • Upright Nikon fluorescence microscope and a dissecting microscope with a high resolution color CCD camera
  • Inverted Nikon TE2000 fluorescence, time lapse, multiple fluorescence, DIC, Phase Contrast, ratio imaging, Fura-2, Prim, CFP/YFP, etc.
  • Leica Fluorescence Stereo Micro/Macrosope
• CONFOCAL MICROSCOPES - Comparison of confocal features - another comparison
  • Spinning Disk Confocal, Perkin Elmer, Ultraview LCS
  • Laser Scanning Confocal Microscope Leica SP2 UV aobs
  • Laser Scanning Confocal Microscope Zeiss 510 meta
  • Laser Scanning Confocal Microscope LeicaTCS-4D
• MISCELLANEOUS
  • Image analysis and quantification workstations - Normaski - Brewster - Snell - Zernike - Young
    • Volocity 5 - Metamorph 7
    • Zeiss 510 - LeicaLCS 2.6 - C-Imaging 6 with deconvolution
    • Adobe Creative Suite - Photoshop - Illustrator - Bridge - ImageReady
    • MS Office 2003/7 - Word - Excel - Power Point - FrontPage
    • QuickTime 7
    • VidEdit
    • VHS video digitization
    • Flatbed scanner with transparency
  • Laser Micro-Dissection and capture
  • Atomic Force Microscope
  • Color solid dye printer: Xerox Phaser 8550
  • Laser jet double sided printer: HP1320
  • Incubator 37 C, 5% CO2 and 95% controlled humidity
  • Refrigerator (4 C) for sample or reagent storage
  • Heated stages, objective heater, 5% CO2
  • Digital Oscilloscope
  • Laser Power Meter (Coherent )
  • Soldering iron
  • Multimeter & Clamp around current meter

Software Resources

• Volocity 4 or 5  3D rendering and classification license on \\Zernike & \\Nomarski  &  \\Snell
• Zeiss LSM 3.2 - full display & analysis software on \\Snell
• Zeiss Image Browser 3.2 - available on CD or download
• LeicaTCS off line with complete analysis  - \\Snell  \\Brewster
• LeicaTCS light  - available on CD or download
• PhotoShop 7.0 or CS to CS4 on \\ Nomarski  \\Zernike  \\Fresnel, \\Hooke, \\Young, \\Snell
• Premiere 6.0 on \\Young
• C-Imaging analysis - \\Nomarski,  \\Young,  \\Snell  and also on acquisition stations: \\Fresnel, \\Hooke, \\Morpheus, \\Euler, \\Shiva, \\Wheatstone, \\Nipkow
• Arcobat 7 or 8
• Winzip 11.0  -  10 computer license
• ThumbsPlus 7 site license

How Tos

Go to How To's

Planned Resources

• 3D Deconvolution - got module for C-Imaging
• Whole embryo imaging

Planned Resources currently shelved

• FCS
• TIRF

		Copyright 2001-09  Dr. M. Chua, Office of Research, School of Medicine, University of North Carolina, Chapel Hill, NC 27599
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