| Notices |
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The Michael Hooker Microscopy Facility is a research
microscopy facility in the Thurston Bowles building at the University of North Carolina, Chapel Hill.
The facility provides standard and advanced digital light microscopy and image
processing resources to users from the UNC Chapel Hill campus on a
fee for use basis.
Instrumentation and instruction are provided to enable users to acquire,
process and analyze images from samples they have prepared, or we can
acquire data with you. Funding assistance may be obtained from the
CTSA Pilot Grant
program to Core Facilities. |
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Information for
new and current users - Users are
required to complete the user
information form and training with facility personnel for each
microscope resource
they wish use, or be assisted with scanning. Please contact Michael or
Neal in order to schedule
training or assisted scanning. All users with
wet or live samples must read this note. |
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| What's New /
Changed |
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What's Down / Scheduled Maintenance - details |
- Apr 12: Multiphoton Ti:Sapphire laser is back in
operation on the Olympus FV1000 con focal microscope.
The chiller unit was replaced.
- Mar 30: Remote desktop access from off campus now
requires connection from your off campus computer using VPN. Please
contact Michael for assistance and details. This only affects
those few who have explicit permission to connect remotely, mostly
to Snell, Nomarski or Schott.
- April or later: Several computers will be taken off
the SOM network because they do not meet minimum security
requirements. This will affect the
LeicaSP2 confocal computer \\Laplace and the Upright Nikon computer
\\Hooke. It will not be possible to access these
computers directly from your computers. With these
machines files will have to be saved or copied to \\Minsky in order
to be able to collect your files over the network. Details
will be posted near these computers closer to the changeover time.
- Oct 15: Leica SP2: A loaner 63X 1.4 NA oil immersion
objective is available while the 63x 1.4 NA UV objective is out for
repair. Please note the loaner objective may not be well corrective for
UV in the z-axis.
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Pictures |
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| Up coming courses/seminars concerning
microscopy and imaging |
- May 07 - June 17 :
Zeiss super resolution fest
- May 15: 4:00 PM "Probing the
Properties of Macromolecular Assemblies in Live
Cells with Single Molecule Imaging and
Manipulation", Maxime Dahan, Ecole Normale
Supérieure, Paris - 1131 Bioinformatics Bldg
- May 17: 10:00 AM Nikon super
resolution light microscopy - Storm & SIM -
G-010 Bondurant Hall -
Seminar flyer -
Nikon flyer
- July 17:
Lasers And
Microscopy meeting - All are invited to attend.
As in previous years the format is a short presentation
of your work. Location 124 Taylor Hall, UNC,
Chapel Hill.
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Location |
- Thurston Bowles building room 6129, 6033a, 6033 &
6123
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| Contacting
Us - Use phone for a quicker reply! |
- Michael 919-843-3268 or text 919-912-9380
- Neal 919-966-7051
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| Widefield Microscopes |
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| Confocal Microscopes |
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Other |
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Computing & Image Processing |
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| Sample preparation, cover slips, test
samples, chambers, calibration, slides, clearing agents, etc. |
| Fluorophore
references (3rd party information)
Spectra |
| Books and articles on microscopy
and imaging |
| Publications |
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 New
Confocal:
An
Olympus FV1000
Multiphoton inverted confocal microscope is located in room 6033.
It features an
environmental box for temperature, gas and humidity control and light
exclusion. An
extensive range of conventional (single photon excitation lasers): 405 nm for UV excited dyes, 440 nm for e.g. eCFP, 488 nm for e.g. FITC,
514 nm for e.g. eYFP, 559 nm for e.g. TexasRed/RFP/dsRed & 633 nm for
e.g. CY5/Alexa633. Motorized x-y stage for tiling or visiting
multiple imaging sites.
MHMF oversight committee request.
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