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Zeiss 510 Meta Laser Scanning Confocal Microscope (\\HERTZ computer)

   

0.  Notices

1.  Scanning

     Fret acquisition and analysis - Sample Fret scans
     Zeiss' manual for the software (v3.2) is available for download from the Zeiss FTP site (pdf format, 3 files). 

2.  The Zeiss 510 Meta Confocal System

 

Objectives  (These are the objectives shipping with the confocal.  Other objectives will be available)
Mag. NA type WD corrections cover slip Immersion xy resolution (ph=1.0) z res (ph=1.0) part no. dic-Wollaston condensor Wollaston  nosepiece position#
10x 0.3 Plan Neo 5.6 mm - - air     44-03-30 dic 44-44-31 (I) II 1
10x 0.45 C-Apochromat 1.82 mm - 0.17 mm (#1.5) water     44-06-38      
20x 0.75 Fluar 660 um - 0.17 (#1.5) air 0.3 um 3.0 um 44 01 45 44-44-39 (III) III 2
40x 1.3 Plan-Neofluar 170 um - 0.17 (#1.5) oil     1056-602 1033-912 (III) III 3*
40x 1.2 C-Apochromomat 290 um cover slip 0.14-0.18 (#1 to #1.5) water     44 00 52 44-44-60 (III) III 5*
63x 1.2 C-Apochromat
(good for deconv)
240 um cover slip 0.15 to 0.17 (#1 to #1.5) water     44 06 68 44-44-69(III)

III

4*
63x 1.4 plan-Apochromat 180 um cover slip  0.17 (#1.5) oil     1113-108 III (1122-909) III 6*
100x 1.4 PlanApochromat 90 um cover slip 0.17 (#1.5) oil     44 07 82 444487 III  
40x 0.80 Acroplan 3 mm   none (ideally) water     40 00 90-01   III  

**** Note that oil lenses are color coded on the nosepiece with a red protector and water lenses correspondingly in blue.

 WD = Is working distance from front of objective to first surface of a #1.5 cover slip

Note: if objectives are changed on the turret then the setup in the LSM 510 Meta must be updated in order to reflect the changed objective parameters.  This is important for correct confocal scanning.  Contact a facility director for assistance.

Links to Zeiss objectives (Note that not all, in fact most, of these objectives are not optimized for confocal microscopy)

Filter sets (widefield epifluorescence - eye viewing only):

position in carousel Zeiss ID Excitation nm beam splitter (dichroic) nm Emission nm fluorophore
2 - - - - DIC polarizer
3 10 450-490 510 515-586 FITC
4 15 546/12 580 590 Rhodamine
5 02 G365 FT395 LP420 DAPI

Viewing Your Images/Image Database/free viewing software/exporting views:

Some notes on the dichroic IDs:

3.  Known Bugs

4.  Displaying Captured Images on your PC computer.  The Zeiss Image Browser (free software)


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Copyright 2001-2014  Dr. M. Chua, Program in Molecular Biology & Biotechnology, School of Medicine, University of North Carolina, Chapel Hill, NC 27599
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Last Updated: 2012-06-01