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Michael Hooker Microscopy Facility (MHMF.ORG)

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Basic scanning on the Zeiss 510 meta Confocal
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Powering Up
Starting the 510 software
Turning on the Lasers
Controlling the microscope for viewing by eye
Setup Scanning Configuration
    Choosing filters for fluorophores in sample
(Configuration window)
    Controlling scanning & detection
(Scan Window)
    Saving your images
Finishing up / Shutting down
Additional Imaging Configurations and Techniques
    - DIC/Transmitted light
    - DIC/transmitted light and double channel fluorescence
    - Sequential scanning (Multi-Track configuration)
    - Sequential scanning with DIC/transmitted light
    - Spectral detection (call Meta on the Zeiss)
    - Sequential Meta with PMT detection
    - Z-series stacks
    - X-Z scanning
    - Reflectance mode
Got You's


Basic scanning on the Zeiss 510 meta Confocal

Powering Up the Zeiss LSM 510 Meta Confocal


Starting the 510 software.  (N.B. LSM 510 software must be running to use the Axiovert 200 microscope)


Turn on the Lasers


Controlling the microscope for viewing by eye (Widefield)


Setup Scanning Configuration - Choosing filters for fluorophores in sample

 


Setting Up Scan Controls - Controlling scanning & detection


Finishing up / Shutting down

ALL USERS MUST FOLLOW SHUT DOWN PROCEDURES CAREFULLY AND COMPLETELY

  1. Select Argon/2 laser unit from the Laser control window
  2. Set Argon/2 laser Output (%) to minimum (25%)
  3. Select "Standby"
  4. If another user is arriving in the next hour or so (see booking page) lasers may be left running.
    If another user is not arriving select Off for all lasers.

  1. Leave the Remote Control switch on
  2. Laser fans will run for about 5 minutes then turn off automatically (a reduction in fan noise will be noticed)
     
  3. Clean up immersion lenses used with lens tissue only
  4. Clean up oil and fluids from stage
  5. Exit Zeiss software
  6. Transfer data to e.g. \\MINSKY\USERS2 (MHMICROSCOPY server) or USB key drive or burn CD, etc.
  7. Log off Windows with "Start" --->  "Shut Down" ---> "logoff username"
  8. Once laser fans have become quiet (automatically powered down) turn Remote Control switch off
  9. Switch off Mercury Arc lamp
  10. Replace microscope dust cover, but avoid covering the hot HBO lamp
  11. Put all used disposable paper products, cans, wrappers, etc. into trash can

The last user that was booked or using the system is responsible for ensuring the system is shutdown.  If you are the last booked user and have cancelled on the same day then you must ensure the system is shut down:


Other Imaging Configurations and Techniques
          For specific help with these scanning options please make an appointment with facility personnel

For specific help with these scanning options please ask facility personnel

Got You's


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Last updated 2014-07-24