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Perkin Elmer Yokogawa Nipkow disk confocal

Introduction:

This confocal system has a Yokogawa multiple lens Nipkow spinning disk scanner (CSU10), allowing it to capture confocal images rapidly with a minimum of excitation light.  Images are captured using a Hamamatsu OrcaER camera driven by the SimpelPCI software from Hamamatsu.  Excitation light is provided by a ~100 mW Krypton/Argon laser, which can excite at 488, 568 & 647 nm.  Imaging of FITC-like, Rhodamine-like, Cy5-like and similar fluorophores, including GFP, Calcium Green & Texas Red can be carried out.  A filter wheel changes emission filters.  A Prior motorized drive is attached to the fine focus knob for software control of z- position.  Transmitted light shutter permits widefield, DIC or phase contrast images to be interlaced with fluorescent image capture.  Acquisition modes can be combinations of z-series, time lapse, &/or multiple fluorophores.

The confocal head is mounted on a Nikon TE2000E inverted microscope.  Analysis software includes the Volocity 3D rendering & quantitation package & C-Imaging (including deconvolution on the \\Nomarski image processing workstation).  Images can be transferred directly over the network.

Notes:

Operating the System

Equipment:

Objectives  (These are the objectives shipping with the confocal.  Other objectives will be available)
Mag. NA type WD corrections cover slip Immersion part no. dic/phase condensor Wollaston   
20x 0.75 S-Fluor 1.0 mm   0.17 (#1.5) air 93127      
40x 1.3 S-Fluor 0.22 mm   0.17 (#1.5) oil 93130      
100x 0.7-1.3mm S-Fluor 0.2 mm NA 0.17 (#1.5) oil        

Filters

Position # Label Fluorophore Filterset Specifics
1 ---- Trans - -
2 UV DAPI Nikon UV-1A  
3 B FITC, GFP Nikon B-2E/C Specifics
4 G Texas Red 96322 TR C109382  
5   CY5 96324M CY5 HQ  
6        
not installed -  CFP 96341 HQ: CY GFP C77389 ex=D436/20 65555, em=HQ480/40 66403
not installed - YFP 96345 HQ: Y GFP C74135 ex=HQ500/20 60341, em=HQ535/30 Chroma 41028

 

Position # Label Fluorophore Filterset Specifics
1 B FITC, GFP Nikon B-2E/C Specifics
2 G Rhodamine Nikon G-2E/C Specifics
3        
4        
5        
6        
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    Last Updated: 2014-07-24