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Nikon TE2000U  Dual mode / Time lapse / Ratio Imaging  Microscope

Introduction:

This high performance widefield microscope system can perform complex and rapid acquisition tasks such as time lapse, fura-2 and other ratio imaging, rapid filter changes, red light DIC with fluorescence (dual mode) and color capture of transmitted light samples.  Optical modes of operations are transmitted light, DIC (Nomarski), Phase contrast & Fluorescence.  A high sensitivity CCD camera as well as an intensified CCD camera are available.  See facility personnel about these functions before using the microscope. 

Book this scope:

Warnings:

Notes:


Powering up: - (if everything is off)

 

Setting up for transmitted light imaging
--> If transmitted illumination is not required then go to "for fluorescence only" below

Setting up for fluorescence light imaging


Dual imaging head setup for Fura-2 and transmitted light imaging

We can setup for Fura-2 & CFP & YFP at your request.  E.g. see this ref. J. Physiology 589, 2437


Open this section only for printing: Acquiring Images Using SimplePCI with the DVC-1412 or DVC-1312

Acquiring Images Using SimplePCI with the DVC-1412 or DVC-1312

Powering down


Equipment Information

Objectives:

Cameras:

Filter Switchers

Filters:

Dual imaging head

        Dichroic wheel positions:

  1. 100% to camera on Morpheus computer
  2. 100% to camera on Wheatstone computer
  3. Fura-2 dichroic for dual mode imaging
    Dual imaging head setup for Fura-2 and transmitted light imaging
  4. 100% to camera on Wheatstone computer

Computers:


Booking Resources
Copyright 2001-2014  Dr. M. Chua, Program in Molecular Biology & Biotechnology, School of Medicine, University of North Carolina, Chapel Hill, NC 27599
Go Back Booking Resources
Questions/comments/problems: Michael Chua

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Last Updated: 2010-07-20