Michael Hooker Microscopy Facility (MHMF.ORG)

Spectra

Dye Spectral Characteristics Viewers (Java is usually required)

• BD Biosciences |  Fluorescent Protein Spectra 
• Olympus
• Probes at Invitrogen
• Molec & Cell Bio at Arizona  -  FRET
• Nexus Solutions
• Salk Institute  -  simplified
• Fluorescent Proteins at MicroscopyU

Microscope Dichroics

• Leica
• Nikon
• Olympus
• Zeiss

Filter Manufacturers

• Chroma
• Omega
• Semrock

Common Dye Characteristics

• Alexa 350
• Dapi
• CFY
• FITC/Fluorocein
• Alexa 488
• CY2
• GFP
• YFP
• Bodipy
• Rhodamine
• Alexa 546
• CY3
• dsRed
• Propidium Iodide
• Texas Red
• Alexa 568
• Alexa 594
• Alexa 633
• CY5
• Alexa 647

• BCECF  pH dye  Molecular Probes
• Fura-2
• FM 1-43
• FM 4-64
• Acroflavin
• Acridine Orange
• ToPro-3

Dyes to avoid

• FITC/Fluorocein - bleaches, pH dependent, long emission tail, bleeds into Rhodamine/Alexa 546/TRITC
• Rhodamine - FRETs with FITC, bleaches easily
• Phycoerythrin - bleaches, excites with 488 nm (FITC)

		Copyright 2001-09  Dr. M. Chua, Office of Research, School of Medicine, University of North Carolina, Chapel Hill, NC 27599
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