| Probe |
Ex (nm) |
Em (nm) |
|
| Reactive and conjugated
probes |
| Fluorescein |
495 |
519 |
FITC; pH sensitive |
| TRITC |
547 |
572 |
TRITC |
| Texas Red |
589 |
615 |
Sulfonyl chloride |
| Alexa Fluor dyes
(Molecular Probes) |
| Alexa Fluor 350 |
346 |
445 |
|
| Alexa Fluor 488 |
494 |
517 |
|
| Alexa Fluor 568 |
578 |
603 |
|
| Alexa Fluor 594 |
590 |
617 |
|
| Alexa Fluor 647 |
650 |
668 |
|
| Cy Dyes (AP Biotech) |
| Cy2 |
489 |
506 |
|
| Cy3 |
(512);550 |
570;(615) |
|
| Cy5 |
(625);650 |
670 |
|
Adapted from Salk Flow Cytometry home page
http://flowcyt.salk.edu/fluo.html
|
| Probe |
Ex (nm) |
Em (nm) |
|
| Nucleic acid probes |
| DAPI |
345 |
455 |
AT-selective |
| SYTOX Green |
504 |
523 |
DNA |
| Propidium Iodide (PI) |
536 |
617 |
|
| TOTO-3, TO-PRO-3 |
642 |
661 |
|
| Cell function probes |
| Fura-2 |
340/380 |
510 |
AM ester. Low/High Ca++, |
| Fluo-3 |
506 |
526 |
AM ester. pH > 6, Ca++ |
| Fluorescent Proteins |
| BCECF |
490 |
535 |
pH |
| eBFP |
380 |
440 |
(Clontech) Quantum yield
0.18 |
| "GFP" |
396,475 |
508 |
Quantum yield 0.77 |
| eCFP |
434 |
477 |
(Clontech) Quantum yield
0.40 |
| eGFP |
489 |
508 |
(Clontech) Quantum yield
~0.60 |
| eYFP |
514 |
527 |
(Clontech) Quantum yield
0.61 |
| Other probes |
| mRFP1 |
584 |
607 |
(Tsien
lab) Quantum yield 0.25 |
| Calcein |
496 |
517 |
pH > 5 |
|