Michael Hooker Microscopy Facility (MHMF.ORG)
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Michael Hooker Microscopy Facility (MHMF.ORG) |
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Please
register (no cost) by sending an email to Wendy Salmon at
| Spring 2007 - An Introductory Guide to the Theory and Practice of Light Microscopy | ||||
| Location: 321 MacNider Time: 9:30 AM Duration: 60-75 minutes Enquiries: Michael Chua or Wendy Salmon | ||||
| Monday - April 16 | Monday - April 23 | Monday April 30 | Monday - May 7 | Monday - June 11 |
| A Brief History of Light Microscopy: Parts of a microscope, modes of imaging, illumination, Kohler, image formation, condenser & objectives, numerical aperture, phase contrast, DIC | Fluorescence Microscopy: Why use it, Jablonski diagram, contrast, resolution, filters+dichroics, immuno labeling | Detectors, Sampling & Digital Images: CCD cameras, PMTs, noise, color/monochrome, sampling, pixel depth, Nyquist criterion, digital image formats, image processing, ethics | Confocal Microscopy: Theory, sensitivity, pinhole, filters, spectral detection, 3D projections & rendering | Advanced Confocal: Live cell imaging, co-localization, bleed through, deconvolution, software. (Not covered FRAP, breaking the light resolution limit) This talk was rescheduled from May 14. |
| Handout: 1-introduction | Handout: 2-fluorescence | Handout: 3-detectors-images | Handout: 4-confocal | Handout:
5-advanced mic-the-very-beginning 39 steps |
| Note: For copyright considerations PDF handouts will only be downloadable from computers located on the UNC Chapel Hill network | ||||
Many thanks to the Carolina Roadmap Executive Committee for providing printing resources for the handouts for this course and to Karen Stone for all her help and support with organization.
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