Michael Hooker Microscopy Facility (MHMF.ORG)
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Michael Hooker Microscopy Facility (MHMF.ORG) |
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| 2006 An Introductory Guide to the Theory and Practice of Light Microscopy | ||||
| Monday - Oct 30 133 MacNider |
Monday - Nov 6 133 MacNider |
Monday Nov 13 G010 Bondurant |
Monday - Nov 20 133 MacNider |
Monday - Nov 27 133 MacNider |
| A Brief History of Light Microscopy: Parts of a microscope, modes of imaging, illumination, Kohler, image formation, condenser & objectives, numerical aperture, phase contrast, DIC | Fluorescence Microscopy: Why use it, Jablonski diagram, contrast, resolution, filters+dichroics, immuno labeling | Detectors, Sampling & Digital Images: CCD cameras, PMTs, noise, color/monochrome, sampling, pixel depth, Nyquist criterion, digital image formats, image processing, ethics | Confocal Microscopy: Theory, sensitivity, pinhole, filters, spectral detection, 3D projections & rendering | Advanced Confocal: Live cell imaging, co-localization,. FRAP, breaking the light resolution limit. multi-channel fluorescence, bleed through. |
| Handout: 1-introduction | Handout: 2-fluorescence | Handout: 3-detectors-images | Handout: 4-confocal | Handouts: 5a-image-processing, 39 steps, 5b-live cell-imaging |
| Note: For copyright considerations PDF handouts are only downloadable from computers located on the UNC network | ||||
| Time 9:30 AM. Location is listed under date. Duration <75 minutes Enquiries: Michael Chua or Wendy Salmon | ||||
(Karen_Stone 966-9431)
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