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Clearing Agents / Procedures

 

Dehydrated it in 70, 95 and 100% EtOH, then methyl salycilate

 

BABB (Sigma, St. Louis, MO) for clearing tissue - benzyl alcohol and benzyl benzoate. RI is 1.54-1.57
    The more dehydrated the tissue is before adding BABB the better

Murray's Clear - 33% Benzyl Alcohol + 67% Benzyl Benzoate

 

Glycerol Mounting Medium with an antifade

DABCO (Giloh and Sedat; Science, 1982)

  • 1% DABCO
  • 10% PBS
  • 90% Glycerol
  • 4% N-Propyl Gallate
  •  

    DABCO (Indig et al., Biochem J. 327:291-8, 1997) a la Dr. Fred Indig

    Hama H, Kurokawa H, Kawano H, Ando R, Shimogori T, Noda H, Fukami K, Sakaue-Sawano A, Miyawaki A. 2011. Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain. Nat Neurosci. 14:1481-8.   |  Overview in Biotechniques

    Ertürk, A., C.P. Mauch, F. Hellal, F. Förstner, T. Keck, K. Becker, N. Jährling, H. Steffens, M. Richter, et al. 2011. Three-dimensional imaging of the unsectioned adult spinal cord to assess axon regeneration and glial responses after injury. Nature Medicine 18:166-171.   |  Overview in Biotechniques 1

    Citifluor  |  TDE  |  Tetrahydrofuran 1


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    Last Updated: 2020-04-28