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Marsico Hall Microscopes Facility (MHMF.ORG)

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Leica Stellaris5 Laser Scanning Confocal Microscope with WLL & UV lasers

Location: 7228A Marsico Hall

Marsico Lung Institute

0.  Notices

1.  Operating the system

2.  The System - see here for details  http://microscopy.unc.edu/confocal/

2.1  Lasers (excitation wavelengths):

2.2  Objectives:

Objectives *  
Mag. NA type WD corrections cover slip Immersion part no. DIC Resolution-xy+ resolution-z 
Objective Wollaston Condenser Wollaston 
5x 0.15 HCX PL Fluotar 13.7 mm        15506224 - - 1.3 um ?
10x 0.40 HC PL Apo 2.7/2.2 mm - #1.5 air 15506407 or 506285 - - 0.49 um 2.6 um 
20x 0.75 HC Plan Apo 620 um -

#1.5

air 15506517 - - 0.26 um 0.65 um
40x 1.25 Plan Apo 350 um corr

0.14 -0.18 mm

glycerol 15506422 E K7 0.17 um 0.31 um
63x 1.4 HC Plan Apo 140 um -

#1.5

oil 15506350 E K10 0.14 um 0.27 um 
L40x 0.8 HCX Apo 3.3 mm U-V-I none water ** 506155 - - 0.24  0.82 

** Dipping lens, not kept on turret  + Approx. resolution pinhole=1 Airy unit 
WD = working distance  DIC = differential interference contrast or Nomarski

2.3  Detection:

2.4  Direct Eye Viewing Fluorescent filters (Widefield, i.e. not confocal)

Epifluorescent cubes used for viewing by eye (not confocal scanning)

 

LCD display

Carousel Position #

Leica cube ID

Leica part

Excitation Color of light

Emission Color

Beam splitter

Typical fluorophores

 

Analyzer

 

(Empty)

 

-

-

 

-

 

LED-405

 

 

15525338

UV 405/60

470/40

455

DAPI, Hoechst

GFP

 

 

15525314

Blue  BP470/40

525/50

495 nm

FITC, eGFP, eYFP, FM 1-43, CY2, Alexa 488

 

RHOD LP

 

 

15525303

Green  BP539/45

   

Texas Red, Rhodamine, CY3, Alexa 555/568/594

 

(SCAN)

 

(Empty)

-

-

-

 

 

 
           

Leica fluorescent cube information | Cube information 3rd party

3.  First time use (new user)

4.  Known Bugs

5.  Viewing Files

8.  Leica confocal Image analysis and processing

  • LAS X core - Good for viewing.  Limited processing.
  • Fiji
  • Volocity

    7.  Links

    8.  Reporting Problems

        Please contact Michael
        Leica Confocal Service and Support
        866-830-0735    8:00 A.M. to 5:00 P.M.
        confocal@leica-microsystems.com
        serial number 820000189
        Note: if you contact Leica directly please let Michael know
        
    Installation date: 2020-10-05

    10.  Dealing with software crashes and Microscope Stand Lock Ups

    11. Applications:

  • Leica LAS X core 3.7 - free off line viewing and exporting
  • http://fiji.sc

  • Booking Resources
    Copyright 2001-2021 Dr. M. Chua, School of Medicine, University of North Carolina, Chapel Hill, NC 27599
    Go Back Booking Resources
    Questions/comments/problems: Michael Chua

     

    Last Updated: 2021-09-09