Overview of the Facility - Mission Statement
Advanced Light Microscopy & Imaging
A Leica-SP2 AOBS confocal upright microscope with UV and visible excitation wavelengths, aotf beam splitter (replaces dichroic filters), detection with spectral
separation, FRAP, FRET
A Zeiss 510 META laser scanning confocal inverted microscope with visible excitation
wavelengths and spectral detection, FRAP, FRET
- A Perkin Elmer Ultraview LCI (Live Cell Imaging) laser spinning disc
confocal microscope using the
Yokogawa dual Nipkow disk confocal head (CS10)
- An Olympus FV1000 inverter confocal microscope. 405, 440, 488,
514, 559, 635 nm & Ti:sapphire (690-1040 nm) lasers. Incubator
enclosure, SIM scanner, x-y motorized stage (March 2011).
- Thermo Atomic Force Microscope
- Leica AS-LMD - Laser Micro-Dissection system
- Inverted widefield microscope, Nikon TE2000U, with transmitted, DIC, phase
contrast, time lapse, ratio imaging, FRET, simultaneous DIC/Fluorescence, DG4
rapid excitation filter changer, Sutter Lambda 10-2 emission filter wheel
changer, DVC1412 cooled CCD & DVC IntensicamII 1412 cameras
- Upright microscope, Nikon Diaphot-SA, with high resolution pixel shifting
color camera (Nikon DXM 1200)
- Upright microscope, Nikon 80i with transmitted, DIC, phase contrast, and
fluorescence illumination, Photometrics QIclick camera, Nikon Elements
- Dissecting microscope, Nikon, shares camera and acquisition system on
- Dissecting fluorescence macroscope, Leica MZ16FA with OrcaER &
Micropublisher 5 cameras, motorized filter changers, z-axis control.
- Inverted fluorescence, transmitted and DIC widefield microscopes, Leica DMIRB,
with MicroPublisher 3.3 megapixel cooled color & OrcaER cooled b/w camera
- 5 PC imaging workstations, 3 high end PCs with 24" hi def monitors for
high resolution analysis and viewing with high performance OpenGL graphics
cards. One workstation with Windows 7 64bit 8GByte RAM + 1GByte ATI
video. Also PC for video capture and
- File server and domain controller, \\MINSKY &
- RAID backup storage: \\ABBE 10.5 TBytes, \\AI 7.2 TBytes, \\MARVIN 3.5
- Web server - http://microscopy.unc.edu/
- FTP server - ftp://220.127.116.11
- Xerox Phaser 8550 solid dye color printer. On network, print to \\Minsky
- HP 1320 b/w laser printer, dual sided. On network, print to \\Minsky
- Nuaire humidity controller incubator
- Refrigerator 4C
- Various heated stages, air flow heater, objective heater, gas curtain,
Tokai Hit stage warmer
- Coherent laser power meter with visible sensor and in the future a UV
- AC In line current meter.
- Digit Oscilloscope 100MHz
- Various software, including C-Imaging 6.0, Metamorph 7.1, Volocity 5,
Volocity License Server, Photoshop CS 2 to 5,
Premiere 6, Slicer, Leica TCS & Zeiss 510 full offline analysis, Voxblast, Acrobat
7, ImageJ, ImageJ 64 bit.
2. Mission Statement - Please note that this list is subject to change
The Michael Hooker Microscopy Facility provides high
performance digital image acquisition, processing and analysis equipment and
know how concentrating on
light microscopy. Multiple modes of imaging are supported including
fluorescence, transmitted, differential interference contrast, phase contrast singly or in
combination. Images may be taken in time lapse mode on
most equipment and also as z-sections on the confocal microscopes and the TE2000
widefield \\Nipkon setup. Staff is available to assist with training, operation,
maintenance and trouble shooting of the equipment. All
users receive training on the use of specific equipment based, when practical, on their prior
knowledge before being allowed to use any of the systems. Training is directed towards
producing knowledgeable and independent users. Sample
preparation, image analysis and file archiving is the users' responsibility, but
advice is offered.
Collaboration with investigators is encouraged. Most use of equipment is charged on a fee
based on units of time used and by the
type of equipment used. Assistance with scanning may be provided at an additional
cost. Users may
come from the UNC research community as well as from other Federal, State,
private and commercial institutions. Walk up assistance and advice is
provided depending on personnel availability.
The facility is currently under the management of Dr. Michael Chua
& Dr. Neal Kramarcy and is located in rooms 6129, 6033a, 6123 and 6033 of
the Thurston Bowles Building in the School of Medicine. Hardware resources are
nominally available from 7 am to 6 pm Monday to Friday to trained users.
After hours use is permitted by validated experienced users. Please make arrangements
with the staff for access to the facility. After hours building access requires
a SOM access card to the Thurston Bowles
building. All users should see:
Please submit this form
on line and also inform facility staff. Please allow up to 2 business days to complete
this process. Plan ahead!
Training in the independent use and proper care of the
microscopes, the associated computer equipment and software, advice in
experimental design and assistance in optimal use of the equipment and image
processing and handling are available. Once trained and approved, users are allowed independent
access to the equipment. Training,
which involves major contact time, is done by appointment.
A World Wide Web booking calendar system at
http://microscopy.unc.edu/booking.htm is provided for
the facility, and is viewable on the world wide web. Authorized users may
make or cancel bookings. World Wide Web
pages concerning usage of, information about and status of the equipment is
located at http://microscopy.unc.edu.
These pages are under constant development and revision.
The facility staff assure that the facility, including
optical, mechanical, software, network and computer components, are
running efficiently and smoothly. Occasionally
small programs are written to assist in the handling of image data,
especially multi-channel, z-series and time series data, and made available to
authorized users of the facility.
Logs of equipment and ancillary computers usage are kept. The information from
these logs is passed on to Program in Molecular Biology & Biochemistry where
accounting personnel prepare
invoices. In accordance with UNC recharge
facility rules all users are billed for time used. PIs are billed
via the usual UNC billing channels.
Staff duties include:
- Ad hoc user problem solving & consultations - we will
answer queries after hours.
- Design of the
- Operation of the
- Thesis committees
- Imaging techniques
- Image acquisition
- Some custom
- Training users
- Image storage
- Image transfer
- Archiving of systems
- Facility Computing:
- Image analysis
- Image processing, 2-D
- Image processing, 3-D
- Image processing,
- Image conversion
- Network integration
- Login accounts - Active Domain & Domain Controllers
- RAID servers and file backups
- Facility Web pages:
- Booking calendars
- Billing information
- Image acquisition
- Image processing
- Interact with the diverse
array of researchers at UNC
- Equipment grant preparation and submission
- Maintenance and repair of facility hardware as much as
practical in order to defray expenses to outside venders and UNC IT
Charges will be based on usage time at a rate dependent on the equipment used.
Amounts are subject to change and are governed by UNC recharge account rules.
- Leica or Zeiss Confocal microscopes 2 three hour sessions - rate per user
- Perkin Elmer spinning disk three hour session - rate per user
- Wide field microscopes with digital camera 90 minute session - rate per
- Solutions, slides coverslips, blank CDs DVD+RW, etc please bring your own.
- Immersion oils, lens tissue, gases provided without direct charge
- DRAQ-5, DAPI in limited quantities.
- Many computers have 4X (or better) DVD writers (~15 minute record time for
4 GBytes) and CV writers for data transfer and archiving
- All computers are networked for file transfer/archiving
- Workstations and most acquisition computers have USB and Firewire
- Accessing a shared computer remotely is free of charge but requires a
username and password
- Usage is billed in 15 minute units. In accordance with standard
accounting practice time units used beyond 1 minute will be charged as a full 15 minute unit.
- Charges are made in order to recover operational costs.
- Capital outlays and cost of workshops are not paid for using these
The facility is on the 6th floor of the Thurston/Bowles building, room 6129.
The Zeiss confocal s in room 6033a. Enter via room 6129. Leica confocal
is in room
6123. Multiphoton confocal microscope is in room 6033 (March 2011). Perkin Elmer spinning disk confocal, room 6129, conventional
fluorescence and transmitted light microscopes room 6129. Office room 6007.
Dr. Michael Chua (director), 6007 Thurston Bowles
- Dr. Neal Kramarcy, 6033a Thurston Bowles
Capital funding for the facility was provided by an anonymous donor. The
Michael Hooker Microscopy Facility is no longer financially supported by the
School of Medicine. Funds derived from usage charges are directed to
operational costs, including service contracts, maintenance, consumables and
training staff. The facility has been awarded extramural equipment funds.
Further information please contact the directors
or see the facility's web site at http://microscopy.unc.edu