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Light Microscopy for Biomedical Research - Workshop September 24 to 28, 2007

The Michael Hooker Microscopy Facility, in collaboration with the North Carolina Roadmap Initiative and the UNC-Chapel Hill School of Medicine, is offering a one week workshop on the use of light microscopy in biomedical research.  The workshop is intended to provide an introduction to the basic concepts of light microscopy and its application to biological research with lectures, hands on sessions and industry involvement.

The 2007 workshop is over.  You may submit your information at Application Form if you wish to be added to the wait list.

Tuition and Tuesday dinner: $700.  A limited number of the seats are reserved for those who are members of groups/labs. that already support the MHMF through specific grants.  For more information contact Michael Chua  lmbr-mc@mhmf.org

Microscopy Facility Workshop - Light Microscopy for Biomedical Research 2007 - This schedule is subject to change
Monday - Sept 24 Location Tuesday - Sept 25 Location Wednesday - Sept 26 Location Thursday - Sept 27 Location Friday - Sept 28 Location
9:00 Intro. - History of Light Microscopy, Modes of imaging, Parts of a Light Microscope - M. Chua

 

9-12

9:00 Talk - Fluorescence: Jablonski diagram, contrast, filters, cross talk - W. Salmon

 

9-12

9:00 Talk - Confocal  Microscopy I - principle, scanning - M. Chua  

8:30-10:30

9:00 Talk - Advanced confocal, 2 photon, spinning disk, colocalization, digital images - M. Chua  

09-10

9:00 Invited Talk - 3-D Image Deconvolution, 3-D Visualization - Katie Buchanan  

9-10:30

10:15 Break 10:30   10:15 Break 10:30   10:15 Break 10:30   10:15 Break 10:30   10:15 Break 10:30  
10:30  Objectives, Kohler illumination, Image formation  

9-12

10:30 Invited Talk - Immuno labeling, cell labeling
Kelly Lundsten
 

9-12

10:30 Confocal II continued - PMT, multichannel, 3d, projections  

10:30-12

10:30 Invited Talk - Fluorescence and FRAP - Chris Baumann  

10:30-12

10:30 Image Deconvolution, Tracking, 3-D rendering LAB

10:30-12:30

11:45 Lunch break   11:45 Lunch break   11:45 Lunch break   11:45 Lunch break   12:30 Lunch break  
12:45 Hands on - parts of a microscope, care, digital acquisition - alignment LAB 12:45 Hands on - Fluorescence LAB 12:45 Hands on  Confocal I -  sensitivity, pinhole, filters, averaging, zoom pixel size z-series, time lapse, simult/sequen. Coloc. LAB 12:45 Hands on Confocal II - multi-channel, FRAP, ) Rendering, 3D, projections
3-D  Deconvolution
LAB 1:30 Invited Talk - Visualization and Representation -  Dr. Russell Taylor 1-2:30

 

15:30 Invited Talk -  DIC/Phase Contrast - Dr. Ted Salmon  

15-17

15:30 Invited Talk -
1. CCD cameras,  noise, color/monochrome  Mike Meade
2. Digital images, sampling, resolution, Nyquist - M. Chua

 

15-17

15:30 Invited Talk - Fluorescent Biosensors for Living Cells - Dr. Klaus Hahn  

15:30-17

15:30 Invited Talks -
0. Light sources
1. Lasers John Miles
2. White light laser Juergen van der Heiden

 

15:30-17

14:30 Hands on - movies, scaling compression, resizing, conversion, format for Power Point LAB

15-17

Finish 5 PM   Finish 5 PM
Dinner 6:30 PM
  Finish 5 PM   Finish 5 PM   Finish 5 PM  

Faculty includes: Ted Salmon, Klaus Hahn, Katie Buchanan, Frank Herbert, John Miles, Larry Kordon, Hilary Hicks, Chris Freson, Dave Tilley, Juergen von der Heiden, Lennie Cleaton, Kelly Lundsten, Janine Babulski, Katie Buchanan, Elise Shumsky, Mike Meade, Chris Baumann, Russell Taylor

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