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An expanded version of this hands on course will be run again in the Fall of 2007

Microscopy Facility Tutorial Workshop - Light Microscopy for Biomedical Research 2006
Monday - Dec 4 Location Tuesday - Dec 5 Location Wednesday - Dec 6 Location Thursday - Dec 7 Location Friday - Dec 8 Location
9:00 Intro.  Brief History of Light Microscopy, modes of imaging, Parts of a Light Microscope - M. Chua TB7033
(9-11) no food permitted in room
9:00 Talk - Fluorescence, why use it, Jablonski diagram, contrast - W. Salmon TB3200
(9-11) food OK
9:00 Talk - Confocal Microscopy - M. Chua TH124
(9-11) food OK
9:00 Talk - Advanced confocal, multichannel fluorescence, 2 photon, spinning disk - M. Chua TB3200
(9-11) food OK
9:00 Talk - CCD cameras, PMT, noise, color/monochrome  - M. Chua TB7033
(9-11:30) no food permitted in room
Break   Break   Break   Break      
10:00  Objectives, Kohler illumination, image formation
11:00 Break
TB7033
(9-11) no food permitted
10:00 Talk - Filters, Immuno labeling, cross talk, live cell imaging - M. Chua TB3200
(9-11) food OK
10:15 Confocal continued - M. Chua TH124
(9-11) food OK
10:00 Talk - Digital images, pixel depth, sampling, formats, pitfalls, resolution, Nyquist - M. Chua TB3200
(9-11) food OK
10:15 Break  
11:15 Walk through of MHMF - details of each machine

 

TB6129
11:00 Hands on - Fluorescence + Kohler review

 

TB6129
10:45 Hands on - confocal I, sensitivity, pinhole, filters, averaging, zoom pixel size
 
11:00 Hands on - digital image handling, calibration, contrast, time-lapse

 

  10:30 Talk - Brief introduction to: co-localization, deconvolution - M. Chua TB7033
(9-11:30) no food permitted in room
12:00 Lunch break   12:00 Lunch break   12:00 Lunch break   12:30 Lunch break   11:30 Lunch break  
13:00 Hands on - parts of a microscope, care, digital acquisition
  13:00 Invited Talk - Klaus Hahn: Fluorescent Biosensors for Living Cells TB3200
(1:00-2:00) food OK
13:00 Hands on - confocal II, z-series, time lapse
 
13:30 Hands on Confocal multi-channel,  FRAP, spectral scanning(?)
 
12:30 Hands on - movies, scaling compression, resizing, conversion, format for Power Point

13:45 Sum up

 
15:00 Invited talk - Ted Salmon: DIC/Phase Contrast TB3200
(3:00-4:00) food OK
14:00 Hands on - time lapse

 

 
~14:30 Tutorial - Rendering, 3D Hands on - 3D projection

TB6129

15:30  Invited talk - Ken Jacobson: Fluorescence and FRAP TB7033
(3:30-4:30) no food permitted in room
14:00 Invited talk - Franck Polleux: Tracing cortical projections TB3200
(2:00-3:00) food OK
                   

 

Room Building Seating  
7033 Thurston Bowles ~25 No food please
3200 Thurston Bowles 50 Food is OK
124 Taylor Hall >80 Food is OK

Contacts: Michael Chua  843-3268, Wendy Salmon  966-7051, Karen Stone  966-9431 Office of Research, School of Medicine

Instructors:
Allan Fanning, Neal Kramarcy, Wendy Salmon, Michael Chua, Juergen van der Heiden

 

http://microscopy.unc.edu/notices

 

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6:30 PM Dinner with 9 registered attendees & organizers W. Franklin St.