Olympus VS200 - Whole Slide Scanner
- The system is located in the Marsico Lung Institute/Cystic Fibrosis
- It is for the use of
researchers in the Marsico Lung Institute division and their colleagues
- All users are
required to receive training or instruction from Michael before using the
- Ultimate permission to use the system is at the discretion of
the CF Center Director
- All users should log in with their own account (mhmicroscopy domain)
- NEW! The Hamamatsu Orca-Fusion camera
has been replaced. Fan vibration on the original camera was causing lateral
vibrations of ~0.5 um peak to peak which affected both the color camera and
the fluorescence camera imaging.
- NEW! The automatic oiler for the 60x
immersion objective has been repaired. Please keep using this
objective. Its performance is still being assessed. Please
report problems to Michael.
- NEW! 6x slide holder: Only one is
available (s/n: 35001739). Z-range limits are set for this slide
holder and hopefully slides will not crash samples into the 40x or 60x
objectives, especially during autofocusing or EFI scanning. Note: 6x
slide holder s/n: 35001667 is not available since it is ~140 um thicker and
the 40x and 60x objectives moves too close holder. Resetting z-movements
limits 140 um lower will be tested as time becomes available on the system.
If you are not going to be doing fluorescence scanning please consider
turning off the Orca-Fusion monochrome camera on top of the scan box and the
Xylis LED light source
before starting the VS200 software.
This may help with the long term life span of these fans.
VS200 booking Calendar is
If you remove
a slide tray with any slides with immersion oil present (60x scans)
belonging to others or to yourself please:
- Blot the 60x objective with lens
tissue before starting your scans.
- Choose this
objective cleaning mode from the home page to
bring the 60x to a position where it is easily cleaned.
- This will help prevent
oil from dripping into the system or onto other slides.
mounting media needs to be well cured. No slides with wet media near lenses.
Clean off excess wet mounting by blotting with a tissue or cutting off with
a razor blade. Slides with mounting media above the level of the cover
slip must not be be scanned.
Failure to do this will result in the scanner being unavailable for cleaning
- Beware: Fluorescent filters without number%. e.g. during
Overview or Settings for higher magnification scans channels such as DAPI
will use light intensity of what was last set. The light knob will
need to be
manually turned on during previews. In contrast, e.g., DAPI 20% sets power
level to 20% and will turn on lamp automatically. Old DAPI, FITC, TRITC, mCherry, CY5, CY7 are being kept for compatibility (in the future
may be removed).
- NEW! Don't open the large white door
of the system while scanning. The weight of the open door causes the internal
frame to flex. Flexing will move the image ~5 um and also change the
- Store scans on the D: drive. In an emergency data may be stored on E: or C:. Avoid
using the C: drive
- Connecting to a server
(mapping to a driver letter) e.g. CF Shared, \\Minsky.med.unc.edu\vs200, \\minsky.med.unc.edu\vs120,
- Olympus recommends not scan directly to network drive shares or mapped
drive letters. Scans are
significantly slower when made over network. Also data
corruption may occur when network slowdowns and disruptions occur.
It is best to scan to D: and copy files later to servers.
- The VS200 software does not work properly when saving to
shares of the form \\minsky.med.unc.edu\VS200
(i.e. network connection without a
- VS200 booking Calendar is
- Face covering for mouth and nose is required.
Gloves are suggested.
- Wash hands before and
after using the system. Soap and paper towels are available at rest rooms
near elevators or at room 7228B. Or use 70% alcohol and paper towels.
- ~70% alcohol is for
gently wiping down (NOT spraying) the keyboard,
mouse, joy stick and table tops
- Ethanol should not be used on optics
- Spray alcohol onto paper towels/kimwipes well
away from system.
- NEVER spray alcohol near or
any part of the system!
- Please remove your slide trays, boxes, samples etc.
from the area
- Dispose of gloves before leaving
- Add institutional requirements when above does not
general hygiene tips for microscopes
- 6 Slide holder - 3" by 1" slides 1.0 mm thick. Covers Slip should
be #1.5 (170 um)
- Transmitted light - LED lamp
- Fluorescence - DAPI, FITC, CY3, CY3.5 (mCherry), CY5, CY7
- Fluorescence - 6 channel DAPI like, FITC like, Rhodamine like, TexasRed
like, CY5 like & CY7 like, slot space available for future upgrade e.g. CFP
- LED Fluorescence light - X-Cite
||GFP, Alexa 488, CY2,
||Opal 570, CY3,
||Alexa 594, Opal 620, Texas Red
||Alexa 633/647, Opal 650, Draq5
||CY7Alexa 750, Opal 750
- CMOS camera fluorescence - Orca
- Color camera for transmitted light/chromogenic sample imaging
- Slide label reader for Bar codes & QR codes
1 Standard immersion
(carbon) oil (RI=1.515)
- OlyVIA 3.2.1 for Windows -
https://www.olympus-lifescience.com/en/software-download/ - Also available
- FIJI for processing, quantitation and analysis
Using the system:
- Quick startup/shut down
guide - ask Michael
- Powering up:
- Power on at bottom right of main bax
- If doing fluorescence power on:
- Camera on top of main box. Please do this gently so as no to
rotate or move the camera.
ligh source - X-Cite Xylis
- At the system browse to \\minsky.med.unc.edu and select desired
- Right click on share and map to a free drive letter
- It is possible to map to UNC servers - see