REQUIRES

SimplePCI
Filter Setting

Light at the specimen

Dichroic filtercube position on the Microscope

AND

DG-4 Filter...

…in DG-4 position #

Default Idle Position

No light

Any

n/a

n/a

WHITE LIGHT

White Light to filtercube turret

 **For single-channel filtercubes ONLY**

UV (DAPI)   

"Open"

1
 

    or

B (FITC, GFP, AlexaFluor 488) 

    or

R (Cy5, AlexaFluor 647 or 633)

DAPI

UV light for excitation of DAPI

M* (multiple excitation)

DAPI
403/12 nm
or
"Open"

4

1

    or

UV (DAPI)

FITC

Excitation for green-emitting fluorophores

M* (multiple excitation)

FITC
492/19 nm or
"Open"

3

1

    or

B (FITC)

Texas Red

Excitation for

red-emitting fluorophores

M* (multiple excitation)

TX RD
572/23 nm
or
"Open"

2

1

    or

G (Texas Red, Rhodamine)

380nm

FURA 380nm excitation

F-2

380nm

2 (3^)

340nm

FURA 340 excitation

F-2

340nm

1 (4^)

manDAPI

White Light to filtercube turret

"Open"

1

manFITC

White Light to filtercube turret

"Open"

1

manRhod

White Light to filtercube turret

"Open"

1

man647

White Light to filtercube turret

"Open"

1

PRIM 470/20

Excitation at 470nm for PRIM

Not currently installed.  See Facility Management.

PRIM 470/20

4

PRIM 410/40

Excitation at 410nm for PRIM

Not currently installed.  See Facility Management.

PRIM 410/40

3

Transmtd  (Uniblitz)

Shutter for transmitted light

When Transmitted light only: "T" dichroic position.  Also see "Setting up for transmitted light imaging" for configuration of transmitted light path.

 With Fluorescence: Set filters for corresponding fluorescence imaging.