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Marsico Hall Microscopes Facility (MHMF.ORG)

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Nikon 80i Upright Microscope

Introduction:

This upright widefield microscope system is intended for transmitted light imaging or fluorescence imaging. Transmitted light imaging uses the green QIclick camera with Windows 10 on the computer called Hamilton.  A gray Hamamatsu OrcaER camera is available for fluorescence using the Windows 7 computer called Farnsworth. Please feel free to discuss camera capabilities and using the microscope with personnel

Book this scope:

Warnings:

Notes:

Powering up: - (if everything is off)

Setting up for transmitted light imaging
--> If transmitted illumination is not required then go to "for fluorescence imaging only" below

Setting up for fluorescence imaging (only if required)

Q-Capture - running the camera

Powering down


Equipment Information

Objectives:

  Mag NA   Feature Condenser Phase WD  
  2x 0.1 PlanApo lambda 2-4x - 8.5 mm  
  4X 0.13 PlanFluor   2-4x PhL 16.5 mm  
  10X 0.3 Plan Apo   0 Ph1 4.0 mm  
  20x 0.75 Plan Apo   0 Ph2 1.0 mm  
  40X 0.75 Plan Apo corr 0 Ph2 0.14 mm Correction collar must be set correctly
  100x 1.4 Plan Apo   0 DIC 0.13 mm  
  100x 1.3 0 Ph3 Not kept on scope

 

Camera:

Filters:

Computer:


Booking Resources
Copyright 2001-2020 Dr. M. Chua, School of Medicine, University of North Carolina, Chapel Hill, NC 27599
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Questions/comments/problems: Michael Chua

Last Updated: 2020-03-10