Macromolecular Interactions Facility (MacInFac) Apprenticeship

With the support of the North Carolina Biotechnology Center and the University of North Carolina at Chapel Hill, the Carolina Roadmap program is pleased to offer an apprenticeship in the Macromolecular Interactions Facility (MacInFac) (http://hekto.med.unc.edu:8080/macinfac/).

The apprenticeship will be taught by Dr. Ashutosh Tripathy, the director of the core.

 

This core provides access to a variety of biophysical methods. These include microcalorimetry, circular dichroism, fluorescence, light scattering, analytical ultracentrifugation and surface plasmon resonance. Equipment available in the Macromolecular Interactions Facility includes a Biacore 2000, Beckman XL-I Analytical Ultracentrifuge, SPEX Fluorolog-3 Research T-format Spectrofluorometer with steady-state and stopped-flow housings and polarization/anisotropy measurement capability, Microcal VP-ITC Isothermal Titration Calorimeter, Microcal VP-DSC Differential Scanning Calorimeter, Applied Photophysics CD Spectropolarimeter, and Wyatt Multi-angle Light Scattering Equipment coupled with a size exclusion column. The capabilities include measurement of affinity, stoichiometry, kinetics and thermodynamics of interactions among proteins, DNA and their cognate ligands. Resources exist for analyzing the biophysical characteristics of molecular weight, shape, and conformations of proteins and DNA; and for exploring bimolecular interactions in real-time.

The program will run from March 26 to April 27, 2007, on the following schedule:
- Mondays and Wednesdays, 11:00-11:50am, in 305 Mary Ellen Jones Building
- Fridays, either 1:00-3:00pm or 3:00-5:00pm, in B30 Wilson Hall

Trainees must be UNC graduate students, fellows, or faculty members. Only 5 trainees can be accommodated, and you must commit to attend all classes and labs. However, we will try to accommodate trainees who are interested in learning only a subset of the techniques. Those who have not taken Biochemistry 151/Chemistry 235, “Macromolecular Interactions,” will be given preference.

There is no cost to attend this program.

If you are interested in joining this program, please reply to Karen Stone at karen_stone@med.unc.edu. Please include the following information: name, role on campus, department and school, and a brief statement about how you will use the instrumentation in your research; please be as specific as you can.

-- 
Karen Stone, MBA
Assistant Director, Carolina Roadmap Program
G060 Bondurant Hall
CB #7000
University of North Carolina at Chapel Hill
Chapel Hill, NC 27599-7000
919-966-9431
fax: 919-966-7564
karen_stone@med.unc.edu
http://www.med.unc.edu/roadmap/welcome.htm